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[maximus] Charlie Electrics(720p) The Oasis(720p) INDIAN THEME HD. Smart,Scary,Comedy,Horror,2014. I can't figure out why my friends from college are bullying me. The Climb is a great film about defying all odds.){ref-type="fig"}). After LC‐MS/MS analysis of the five fractions, phosphopeptides containing a phosphorylation site associated with PLK1 were determined. Figure [1](#febs14001-fig-0001){ref-type="fig"}(E) shows the plot of the relative abundance of the five fractions against the relative abundance of the identified phosphopeptides in total. Phosphopeptides derived from STK38 were enriched by LC‐MS/MS analysis of the five fractions. Therefore, fraction 5, which contains STK38 and phosphorylated form of STK38, was identified to be the fraction enriched with the maximum amount of phosphorylated STK38. Figure [1](#febs14001-fig-0001){ref-type="fig"}(F) shows the map of detected STK38 peptides from the five fractions. Analysis of the five fractions revealed that fraction 5 is the fraction in which the maximum amount of STK38 is phosphorylated.
Phosphorylation sites of STK38 are associated with mitotic events {#febs14001-sec-0004}
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To identify the possible STK38 kinase(s), the enrichment of *in vitro* phosphorylated STK38 was analyzed by Western blotting. Figure [2](#febs14001-fig-0002){ref-type="fig"}(A) shows the enrichment of the STK38 phosphorylation induced by Myt1. Among the STK38 kinases, Myt1 is the most suitable STK38 kinase. Then, the STK38 phosphorylation induced by Myt1 was confirmed by *in vitro* kinase assay (Fig. [2](#febs14001-fig-0002){ref-type="fig"}(B)). In *in vitro* kinase assay, STK38 could be phosphorylated by its own kinase, Myt1, indicating the possibility of autophosphorylation. Figure [2](#febs14001-fig-0002){ref-type="fig"}( 0b46394aab
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